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Thursday, July 23, 2020 | History

2 edition of In-situ aquifer restoration of chlorinated aliphatics by methanotrophic bacteria found in the catalog.

In-situ aquifer restoration of chlorinated aliphatics by methanotrophic bacteria

In-situ aquifer restoration of chlorinated aliphatics by methanotrophic bacteria

project summary

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Published by U.S. Environmental Protection Agency, Robert S. Kerr Environmental Research Laboratory in Ada, OK .
Written in English

    Subjects:
  • Aquifers -- Research

  • Edition Notes

    Statementby Paul V. Roberts ... [et al.]
    ContributionsRoberts, Paul V, Robert S. Kerr Environmental Research Laboratory
    The Physical Object
    FormatMicroform
    Pagination8 p.
    ID Numbers
    Open LibraryOL17933062M

    for the in situ quantification of microbial activities in PHC-contaminated aquifers (21, 36, 41). In a PPT, a test solution that contains a nonreactive, conservative tracer and one or more reactive solutes (reactants) is injected (pushed) into the aquifer through an existing well. During an initial incubation. evaluation of in-situ methanotrophic bioremedia tion for contaminated groundwater. stanford university. in situ restoration of chlorinated aliphatics by methanotrophic bacteria. stanford university. 6/1/ drinking water wells & aquifer characteristics in three mile radius of bendix.

      Abstract. In sediments, methane-oxidizing bacteria live in opposing gradients of methane and oxygen. In such a gradient system, the fluxes of methane and oxygen are controlled by diffusion and consumption rates, and the rate-limiting substrate is maintained at Cited by: Enrichment and Molecular Detection of Denitrifying Methanotrophic Bacteria of the NC10 Phylum Katharina F. Ettwig,* Theo van Alen, Katinka T. van de Pas-Schoonen, Mike S. M. Jetten, and Marc Strous† Department of Microbiology, Institute for Water and Wetland Research, Radboud University Nijmegen, NL ED Nijmegen, The Netherlands.

      Microcosm preparation, quantitation of benzene and isolation of organisms. Microcosms were prepared in replicates of 10, in mL serum bottles with 20 mL of groundwater from wells d and W6i, with benzene at nominal concentrations of 1, 10, and mg L − microcosms contained 19 mL of medium (Fahy et al., ), 1 mL of groundwater from well W18i and benzene at Cited by: In Situ Air Stripping: Cost Effectiveness of a Remediation Technology Field Tested at Savannah River Integrated Demonstration Site, LA-UR DOE, In Situ Vitrification, Technology Transfer Bulletin, Prepared by Battelle Pacific Northwest Laboratories for DOE, Richland, WA. DOE,


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In-situ aquifer restoration of chlorinated aliphatics by methanotrophic bacteria Download PDF EPUB FB2

EPA/// July IN-SITU AQUIFER RESTORATION OF CHLORINATED ALIPHATICS BY METHANOTROPHIC BACTERIA by Paul V. Roberts, Lewis Semprini, Gary D. Hopkins, Dunja Grbic-Galic, Perry L McCarty, and Martin Reinhard Research Staff: Constantinos V.

Chrysikopoulos, Mark E. Dolan, Franziska Haag, Thomas C. Harmon, Susan M. Henry, Robert A. Johns, Nancy A. Lanzarone. United States Environmental Protection Agency Robert S. Kerr Environmental Research Laboratory Ada, OK Research and Development EPA//S/ Aug.

4>EPA Project Summary In-Situ Aquifer Restoration of Chlorinated Aliphatics by Methanotrophic Bacteria Paul V. Roberts, Lewis Semprini, Gary D. Hopkins, Dunja Qrbic-Qalic, Perry L. McCarty, and Martin Reinhard This protect. In-situ aquifer restoration of chlorinated aliphatics by methanotrophic bacteria Author: Wayne C Downs ; Paul V Roberts ; Robert S.

Kerr Environmental Research Laboratory. Get this from a library. In-situ aquifer restoration of chlorinated aliphatics by methanotrophic bacteria: project summary.

[Paul V Roberts; Robert S. Kerr Environmental Research Laboratory.;]. Chlorinated hydrocarbons removal efficiencies in active methanotrophic bacterial landfill soil cover were found to be >57%, while a recent study demonstrated in situ removal efficiencies of up to Author: Robin Brigmon.

In: Roberts PV, Semprini L, Hopkins GD, Gribic-Galic D, McCarty PL, Reinhard M (eds) In-situ aquifer restoration of chlorinated aliphatics by methanotrophic bacteria.

US Environmental Protection Agency, Robert S. Kerr Environmental Research Laboratory, Ada, OK, EPA Report EPA///, pp. In Situ Aquifer Restoration of Chlorinated Aliphatics by Methanotrophic Bacteria. Technical Report EPA // U.S. Environmental Protection Agency, R.S. Kerr Environmental Research Laboratory, Ada, OK.

Chapter or Article in Book or Proceedings Atlas, R.M. “Bioremediation of Fossil Fuel Contaminated Soils.”. Kate M. Campbell, in Handbook of Water Purity and Quality, In Situ Techniques. In situ bioremediation is an attractive option for groundwater with lower contaminant concentrations because the treatment occurs directly in the subsurface aquifer.

Ideally, in situ bioremediation only requires injection of an electron donor to stimulate activity of indigenous dissimilatory metal reducing or. In situ methane oxidation in groundwater by using natural-gradient tracer tests.

In-situ aquifer restoration of chlorinated aliphatics by methanotrophic bacteria. Research report, 1 May Methanotrophic bacteria are ubiquitous. The growth of type II bacteria appears to be favored in environments that contain relatively high levels of methane, low levels of dissolved oxygen, and limiting concentrations of combined nitrogen and/or copper.

(TCE) by Methanotrophic Bacteria in a Saturated Laboratory Column of Aquifer Material,” Water Science and Technology, Vol. 21, (). P.V. Roberts, G.D.

Hopkins, D.M Mackay, and L. Semprini, “A Field Evaluation of In-Situ Biodegradation of Chlorinated Ethenes: Part I, File Size: KB. In situ stimulation of methanotrophic bacteria has been considered as a methodology for aquifer remediation.

Chlorinated aliphatic hydrocarbons such as trichloroethylene are fortuitously oxidized by the methane monooxygenase produced by methanotrophic bacteria. Experimental results are presented that indicate that both colloidal suspensions. In Situ Aquifer Restoration of Chlorinated Aliphatics by Methanotrophic Bacteria, EPA///; NTIS: PB EPA, Innovative Technology - Glycolate Dehalogenation, EPA, OSWER, Washington, DC, Directive FS.

Vinyl chloride (VC) is a carcinogen generated in groundwater by reductive dechlorination of chloroethenes. Under aerobic conditions, etheneotrophs oxidize ethene and VC, while VC-assimilators can use VC as their sole source of carbon and energy.

Methanotrophs utilize only methane but can oxidize ethene to epoxyethane and VC to by: In situ biostimulation of indigenous methane-oxidizing bacteria in the field by adding methane and other nutrients can enhance the performance of co-metabolic biodegradation of halogenated aliphatics.

All the primary substrates used for biostimulation must be safe and by: One month later, the NC10 bacteria had become the dominant bacteria, and the level of enrichment was about 70% after 7 months.

In the small rods (ca. to 1 by to μm) the 4′,6′-diamidinophenylindole (DAPI) signal was concentrated in the center of each cell, and the cells occurred both as aggregates and as single cells (Fig Cited by: Methanotrophs: promising bacteria for environmental remediation for in situ bioremediation of contaminated soil and water (III)species by methanotrophic bacteria (Methylococcus capsulatus Bath), as the Cr(III) is insoluble and tends to get precipitated at high pH.

Semprini, L. and P.L. McCarty, "Comparison Between Model Simulations and Field Results for In-situ Biorestoration of Chlorinated Aliphatics: Part 1, Biostimulation of Methanotrophic Bacteria," Ground Water, v.

29, no. 3, (). In-Situ Aquifer Restoration of Chlorinated Aliphatics by Methanotrophic Bacteria. U.S. Environmental Protection Agency Technical Report, EPA //, R. Methanotrophic bacteria are absolutely vital for the global carbon cycle and to carbon sequestration, as they constitute the largest known biological methane sink.

Methanotrophs are also central to the bioremediation and biofuel development goals of the DOE. To date, only two methanotrophic bacteria have undergone complete genome sequencing, and only one sequence is available to.

Conventional pump-and-treat systems extract relatively large volumes of water with relatively low contaminant concentrations. Because of geologic complexity and slow rates of contaminant desorption and dissolution, these systems must displace many pore volumes of aquifer water to flush out contaminants, as explained in Chapter tional pump-and-treat systems thus are inherently.methanotrophic bacteria from aquatic environments.

Previ-ously, the process of screening isolated methanotrophs as TABLE 1. Location ofmethanotrophs on Percoll gradients Strain Type Density, g/ml (mean + SD)" Source or Methylomonas albus BG8 1 ± (5) 16 Methylobacter capsulatus Y I ± (3) Box provides an example of data gathered to confirm a biological reaction process, in situ bioremediation of chlorinated solvents using methanotrophic bacteria.

This example can serve as a model for other field tests of biological and chemical reaction processes.